Acyl-CoA hydrolysis by the high molecular weight protein 1 subunit of yersiniabactin synthetase: Mutational evidence for a cascade of four acyl-enzyme intermediates during hydrolytic editing

Resolution of the fatty acid reductase from Photobacterium phosphoreum into acyl protein synthetase and acyl-CoA reductase activities. Evidence for an enzyme complex.

The biosynthesis of long chain aliphatic aldehydes for light emission in luminescent bacteria is catalyzed by a fatty acid reductase in a reaction dependent on ATP and NADPH. Evidence has now been obtained which demonstrates that this reaction consists of two distinct steps: 1) activation of fatty acid and 2) reduction of a fatty acyl intermediate to aldehyde. Fatty acid reductase catalyzed the...

Acyl-CoA dehydrogenases and acyl-CoA oxidases

Acyl-CoA dehydrogenases and acyl-CoA oxidases are two closely related FAD-containing enzyme families that are present inmitochondria andperoxisomes, respectively. They catalyze the dehydrogenation of acyl-CoA thioesters to the corresponding trans-2-enoyl-CoA. This review examines the structure of medium chain acyl-CoA dehydrogenase, as a representative of the dehydrogenase family, with respect ...

Acyl CoA binding protein and cholesterol differentially alter fatty acyl CoA utilization by microsomal acyl-CoA:cholesterol transferase by

The maize high-lysine mutant opaque7 is defective in an acyl-CoA synthetase-like protein.

Maize (Zea mays) has a large class of seed mutants with opaque or nonvitreous endosperms that could improve the nutritional quality of our food supply. The phenotype of some of them appears to be linked to the improper formation of protein bodies (PBs) where zein storage proteins are deposited. Although a number of genes affecting endosperm vitreousness have been isolated, it has been difficult...

An enzymic cycling method for the determination of free fatty acids with acyl-CoA synthetase and acyl-CoA hydrolase.

A method for measuring free fatty acids by enzymic cycling is described. Free fatty acids are converted to acyl-CoAs by acyl-CoA synthetase, then the acyl-CoAs are hydrolyzed back to the free fatty acids by acyl-CoA hydrolase in a cyclic fashion. The amounts of AMP produced during this cyclic reaction are determined from the absorbance at 340 nm in the presence of AMP deaminase and glutamate de...